2PE-STED Microscopy with a Single Ti: Sapphire Laser for Reduced Illumination
نویسندگان
چکیده
We reported a new effective approach to carry out two-photon excitation stimulated emission depletion (2PE-STED) microscopy using a single Ti:sapphire laser system. With an acoustic-optic Bragg cell, the modulated-CW 2PE STED microscope had the benefits of both CW and pulse approaches: lower input power, simple optical scheme and no complicated synchronization. Additionally, it also took advantages of fluorescence yield increasing. The sub-diffraction-limit resolution was demonstrated using ATTO 425-tagged clathrin-coated vesicles.
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عنوان ژورنال:
دوره 9 شماره
صفحات -
تاریخ انتشار 2014